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sgrna scaffold homologies  (New England Biolabs)
96
New England Biolabs sgrna scaffold homologies
Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The <t>sgRNA</t> expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short <t>homologies</t> where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.
Sgrna Scaffold Homologies, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sgrna scaffold homologies - by Bioz Stars, 2026-03
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sgrna scaffold  (Addgene inc)
95
Addgene inc sgrna scaffold
Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The <t>sgRNA</t> expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short <t>homologies</t> where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.
Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna scaffold/product/Addgene inc
Average 95 stars, based on 1 article reviews
sgrna scaffold - by Bioz Stars, 2026-03
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sall1a sgrna synthego modified ez scaffold  (Synthego Inc)
90
Synthego Inc sall1a sgrna synthego modified ez scaffold
Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The <t>sgRNA</t> expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short <t>homologies</t> where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.
Sall1a Sgrna Synthego Modified Ez Scaffold, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sall1a sgrna synthego modified ez scaffold/product/Synthego Inc
Average 90 stars, based on 1 article reviews
sall1a sgrna synthego modified ez scaffold - by Bioz Stars, 2026-03
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scaffold guide rna  (Addgene inc)
93
Addgene inc scaffold guide rna
Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The <t>sgRNA</t> expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short <t>homologies</t> where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.
Scaffold Guide Rna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/scaffold guide rna/product/Addgene inc
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scaffold guide rna - by Bioz Stars, 2026-03
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px601 saucas9  (Addgene inc)
93
Addgene inc px601 saucas9
Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The <t>sgRNA</t> expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short <t>homologies</t> where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.
Px601 Saucas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px601 saucas9/product/Addgene inc
Average 93 stars, based on 1 article reviews
px601 saucas9 - by Bioz Stars, 2026-03
93/100 stars
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Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The sgRNA expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short homologies where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.

Journal: G3: Genes | Genomes | Genetics

Article Title: Near 100% efficient homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans

doi: 10.1093/g3journal/jkaf118

Figure Lengend Snippet: Plasmid maps. a) Marker-guide plasmids for HYGR , NATR , NEOR , and amdS (pBHM2616-2619). Each marker is driven by the ACT1 promoter and terminated by the TRP1 terminator. The sgRNA expression is driven by the U6 promoter and terminated by 6 Ts (not shown); a stuffer sequence includes the Bpl I restriction site for cloning. Arrows indicate primers used for fusion PCR with red tails indicating short homologies where applicable. As examples: pBHM2617 specifies P20 and P22, which were used for the ADE2 deletion cassette with homology arms; pBHM2619 includes primers for fusion PCR to generate a URA5 marker-guide deletion cassette with homology arms (Step 1A: M13F + P29, Step 1B: P30 + P19, and Step 3: P31 + P32). b) Tag-guide-marker plasmids for NATR , NEOR , and amdS (pBHM2660-2662). Each marker-guide portion is as described in (a), and the tag portion includes a linker followed by codon-optimized mNeonGreen (mNG) as well as CBP and 2xFLAG (separated by short linkers; not shown) with a terminator from EST2 . Note that because the amdS gene also contains a Bpl I recognition site (not shown), its use is limited to the fusion PCR method.

Article Snippet: Alternatively, cloning can be performed as follows: after digesting the plasmid with Bpl I, the break can be bridged with a 60 bp ssDNA oligo containing the desired 20 bp target sequence flanked by U6 and sgRNA scaffold homologies (see NEB #E2621).

Techniques: Plasmid Preparation, Marker, Expressing, Sequencing, Cloning